Quantitative DIA-based proteomic analysis of isolated lysosomes (Lyso-IP) and whole-cell extracts (WCL) from VPS35[D620N] knock-in MEFs. The dataset includes six biological replicates comparing cells treated with the LRRK2 inhibitor MLi-2 (100 nM, 2 h) against DMSO vehicle controls. Expression of the 3XHA-TMEM192 lysosomal tag was determined by genotyping PCR and verified via anti-HA immunoblotting. Data acquired on an Orbitrap Exploris 480 and processed using DIA-NN 1.8.1. ASAP Team: Alessi Dataset Name: alessi-mefs-ms-p-vps35-d620n-dmso-mli2, v1.0 Principal Investigator: Dario Alessi Co-Principal Investigator: Suzanne Pfeffer Contributors: Lam, Pui Yiu; Taylor, Matthew Sample Summary: Resources and Additional Information Protocols GitHub: NA Protocols: https://dx.doi.org/dx.doi.org/10.17504/protocols.io.kxygxzrokv8j/v1 Dataset DOI: https://doi.org/10.5281/zenodo.18476410 Associated Publications: NA CURTAIN: IP: https://curtain.proteo.info/#/28734c66-5baa-458e-8135-9305a7c7bf2d WCL: https://curtain.proteo.info/#/31fa4407-00f5-4331-877c-7588c9eb0004 CRN Cloud GitHub: https://github.com/ASAP-CRN CRN Cloud DOI: https://doi.org/10.5281/zenodo.8384742 CRN Cloud Data Dictionary:...
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Quantitative DIA-based proteomic analysis of lysosomes (Lyso-IP) from VPS35[D620N] Mouse Embryonic Fibroblasts (MEFs) with LRRK2 inhibition is published on ASAP CRN Cloud.