Bulk RNA-seq of neurological controls and Parkinson's disease brainsHuman post-mortem brain tissue from donors with Lewy Body pathology and neurologically healthy controls was sourced from the Cambridge Brain Bank under London–Bloomsbury Research Ethics Committee (REC reference no. 16/LO/0508). Donors with Lewy Body pathology (n=25) had a clinical diagnosis of Parkinson's disease and were compared to an age- and sex-matched group of neurologically healthy control donors (n=18) who had no history of neurological illness. A neuropathologist assessed all cases for Lewy Body pathology Braak staging, co-existing proteinopathies and other pathologies. Fresh frozen tissue was sampled from SN, PUT, AMY, and PFC at the level of Brodmann area 46 (PFC). A 3 mm3 tissue piece was cut from the same tissue block that had been used for snRNA sequencing and disrupted using a Tissuelyser (Qiagen). A steel bead and RLT buffer with -mercaptoethanol was added to the Tissuelyser and shaken at 30 Hz for 2 minutes. Total RNA was isolated from the disrupted tissue using the RNeasy Mini Kit (Qiagen; RRID). The sequencing libraries were then generated using Illumina TruSeq Stranded mRNA library prep kit (with poly-A selection) and sequenced on a NovaSeq6000 or Novaseq X...Access requires approval by the data custodian. The collection's description and structure are public; querying the data requires an approved request.Bulk RNA-seq of neurological controls and Parkinson's disease brains is published on ASAP CRN Cloud.