Single-nucleus RNA-seq of brain regions from healthy and Parkinson's disease individualsWe performed single nuclei RNA sequencing of postmortem PD and control brain tissue of four different brain regions (substantia nigra, putamen, amygdala, and prefrontal cortex). Briefly, 8500 nuclei were FACS sorted from approximately 2 mm3 of tissue. Single nuclei RNAseq libraries were prepared using 10X genomics platform for single cell 3' sequencing. Sequencing libraries are prepared by fragmentation, end repair and a tailing followed by sample index PCR. To process the data, FASTQ files were processed using Cell Ranger, aligning and quantifying for hg38. Intronic reads were also quantified. ASAP Team: Jakobsson Dataset Name: jakobsson-pmdbs-sn-rnaseq, v2.1 Principal Investigator: Johan Jakobsson Co-Principal Investigator: Roger Barker Contributors: Jakobsson, Johan; Barker, Roger; Gale Hammell, Molly; Kirkeby, Agnete; Garza, Raquel; Sharma, Yogita; Tam, Oliver; Wunderlich, Cole; Forcier, Talitha; Jones, Jo; Curle, Annabel; Quaegebeur, Annelies; Adami, Anita; Wijesinghe, Sasvi; Jönsson, Marie; Kouli, Antonina; Atacho, Diahann Sample Summary: Resources and Additional Information Protocols GitHub: https://github.com/mhammell-laboratory/ASAP_CRN_data_analysis_scripts Protocols:...Access requires approval by the data custodian. The collection's description and structure are public; querying the data requires an approved request.Single-nucleus RNA-seq of brain regions from healthy and Parkinson's disease individuals is published on ASAP CRN Cloud.